Evaluation of Fermentability of Whole Soybeans and Soybean Oligosaccharides by a Canine In Vitro Fermentation Model

Soybean oligosaccharides (OS) have been recognized as a prebiotic that can be fermented in the colon, resulting short-chain fatty acid (SCFA) production used an energy source for colonocytes, supporting cell differentiation and gut health. The objective was to determine effects of WSBOS on vitro fermentation, using dog feces inoculum. Treatments included total dietary fiber (TDF) residues from WSB, soybean hulls (SH), pea (PF), beet pulp (BP), well WSB TDF residue plus OS (WSBOS) raffinose, stachyose, verbascose (WSBRSV). Fresh fecal samples were collected dogs maintained anaerobic conditions until substrate inoculation. Test tubes containing sources inoculum incubated 4, 8, 12 h at 39 °C. Organic matter disappearance (OMD), pH, SCFA measured. WSBRSV had greater (p < 0.05) OMDs than BP. Butyrate greatest (294.7 µmol/g) (266.1 µmol/g), followed by BP (130.3 (109.2 lowest PF (44.1 µmol/g). WSBOS, PF. In conclusion, has potential demonstrating butyrate canine fermentation model due both WSB. Further animal feeding studies are needed appropriate amount diets.